Dear All: I would like to know if there are techniques to minimize the perturbation caused by tissue contamination, for example, normal lung tissue contamination on a breast metastasis. What is the best strategy to deal with this problem? I think this should be dealt with at the "low" level normalization process, please help. Best regards Hao Liu, Ph. D
Hao Liu wrote: > > I would like to know if there are techniques to minimize the perturbation > caused by tissue contamination, for example, normal lung tissue > contamination on a breast metastasis. What is the best strategy to deal > with this problem? > > I think this should be dealt with at the "low" level normalization > process, please help. I think it should be dealt with at the level of tissue extraction, i.e. use microdissection. Best wishes Wolfgang -- -- ------------------------------------- Wolfgang Huber Division of Molecular Genome Analysis German Cancer Research Center Heidelberg, Germany Phone: +49 6221 424709 Fax: +49 6221 42524709 Http: www.dkfz.de/abt0840/whuber
hi! Wolfgang: I actually talked with some very good pathologists, according to them even microdissected sample (cancer tissue) could have around 20% contamination from surrounding tissue. Your answer to my question makes me wonder if I heard is true, can someone help to clarify this issue? Thanks Best Hao On Mon, 3 May 2004, Wolfgang Huber wrote: > > > Hao Liu wrote: > > > > I would like to know if there are techniques to minimize the perturbation > > caused by tissue contamination, for example, normal lung tissue > > contamination on a breast metastasis. What is the best strategy to deal > > with this problem? > > > > I think this should be dealt with at the "low" level normalization > > process, please help. > > I think it should be dealt with at the level of tissue extraction, i.e. > use microdissection. > > Best wishes(Continue reading)
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