24 Jul 2012 16:12
edgeR: design matrix for different condition
Dear the edgeR community, Good day. I'm analyzing my RNA-Seq experiment with edgeR. My study has 2 different genotypes (treeHS, treeLS) and time of treatment (0H, 3H, 24H, 96H). I first assumed that the treatment effect will be the same for each genotype and I have the design matrix as: design <- model.matrix(~tree+treat) > design (Intercept) treat03H treat24H treat96H treeLS 1 1 0 0 0 0 2 1 0 0 0 0 3 1 1 0 0 0 4 1 1 0 0 0 5 1 0 1 0 0 6 1 0 1 0 0 7 1 0 0 1 0 8 1 0 0 1 0 9 1 0 0 0 1 10 1 0 0 0 1 11 1 1 0 0 1 12 1 1 0 0 1 13 1 0 1 0 1 14 1 0 1 0 1 15 1 0 0 1 1(Continue reading)
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