RE: Regarding primary antibody binding in western blot

I have also observed people probing multiple PVDF blots separated by a nylon mesh to prevent the blots from
sticking to each other, in a box that is just larger than the blot itself.  I thought that was a neat idea.  You
can try that way too. 
Jay

-----Original Message-----
From: methods-bounces@...
[mailto:methods-bounces@...] On Behalf Of Irit Rappley
Sent: Wednesday, May 16, 2012 1:34 PM
To: divya teja
Cc: methods@...
Subject: Re: Regarding primary antibody binding in western blot

Hi Divya,

Yes, you can certainly put 2 membranes in the same box with your primary antibody. Just make sure that the
membranes are back to back, so that the protein side of each membrane is facing outward to the antibody solution.

Good luck!
Irit

On May 16, 2012, at 6:03 AM, divya teja wrote:

> hello,
> 
> Can I use 2 PVDF  membranes at the same time in single box containing 
> primary antibody in 10ml 1x PBST while doing western blot?
> 
> Will the primary antibody bind properly or gets probed properly, if I 
> use 2 membranes same time in the single box????????