RE: Overgrown (3day) LB plates ok to use?

If they were antibiotic resistance plates, they are probably useless and full of satellite colonies.
Jay

-----Original Message-----
From: methods-bounces@...
[mailto:methods-bounces@....indiana.edu] On Behalf Of David Liu
Sent: Monday, August 06, 2012 7:39 PM
To: methods@...
Subject: Overgrown (3day) LB plates ok to use?

I left a replica LB Amp plate in the 37C over the weekend, and I'd like to know if they're still good to
use.  I've started liquid cultures but if they grow, can I use the minipreps without resequencing the
whole plasmid? It's a replica plate, so I don't necessarily need single colonies, and satellite colonies
are not an issue.  I am primarily concerned about spontaneous mutations that may have
accumulated.  Any ideas? _______________________________________________
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