qPCR NEWS - October 2010 - focus on digital PCR

qPCR NEWS - October 2010 - focus on digital PCR
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Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

NEW - Copy Number Variations  => http://digital-PCR.gene-quantification.info
qPCR Symposium USA in November 2010
qPCR Application Workshops  -  and more ... ... ...

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Digital PCR (dPCR) is a refinement of conventional PCR methods that
can be used to directly quantify and clonally amplify nucleic acids
(including DNA, cDNA, methylated DNA, or RNA). The key difference
between dPCR and traditional PCR lies in the method of measuring
nucleic acids amounts, with the former being a more precise method
than PCR. The main principle of digital PCR is that a single sample is
split into many fractions, all of which are subsequently analysed by a
standard PCR method. The sample is fractionated by the simple process
of dilution so that each fraction contains approximately one copy of
DNA template. This separation allows a more reliable collection and
sensitive measurement of nucleic acid amounts. The other major feature
of digital PCR is that data are recorded as positive or negative (i.e.
generation of an amplification product or not). Hence, the individual
readout signals are qualitative or ‘digital’ in nature. The method has